In the distant future...Vampires rule the night, but their numbers are dwindling. With huge bounties on their heads, a class of hunters has emerged, Bounty Hunters. One hunter is unlike all the others. He is a Dunpeal — a half human, half vampire. At war with himself, feared by all, tortured and alone, he is... Vampire Hunter D. — from introD, the infamous dunpeal outcast and renowned vampire hunter has fought for the grudging acceptance among humans through his prowess at hunting the creatures of the night. He is called upon to locate Charlotte Elbourne, the lovely daughter of an affluent family who apparently has been kidnapped by a powerful vampire named Meier Link.Charlotte's father offers a large bounty for her return, be she dead or alive, a task D willingly accepts, even with the notorious Marcus brothers Borgoff, Kyle, Nolt & Grove and their compatriot Leila, seeking the prize as well.As the sun sets, the hunt is on, but as the chase evolves it becomes more and more apparent that some sinister evil lurks in the shadows, secretly plotting and manipulating the hunters' every move. An insidious trap is set, and with the tables turned the hunters become the hunted!

During a fiercely hot summer in Sotoba, a peaceful and quiet village with a population of 1300 people, a series of bizarre deaths begin to occur. At the same time, a strange family moves into a long abandoned mansion in the region. Hospital dean Toshio Ozaki cannot figure out the cause of death in the deceased, initially suspecting an epidemic. As investigations continue between the hospital staff and the residents, it turns out that the village is being attacked by vampires.

10,000 years in the future, the world has become a very different place; monsters roam the land freely, and people, although equipped with high-tech weapons and cybernetic horses, live a humble life reminiscent of millennia past.A small hamlet is plagued by monster attacks, living under the shadow of rule by Count Magnus Lee, a powerful vampire lord who has ruled the land for thousands of years. When a young girl is bitten by the Count and chosen as his current plaything, she seeks out the help of a quiet wandering stranger, D. It so happens that D is a renowned vampire hunter, and he takes it upon himself to cut through the Count's many minions, and put an end to the his rule.

Komori Yui is a positive-thinking girl who nevertheless is troubled by seeing spirits and experiencing poltergeist phenomena. In her second year in high school, she transfers to a new school—a night school for entertainers and celebrities—due to her father’s work. There are rumours that vampires exist among the student body, and Yui ends up living with the six sadistic Sakamaki vampire brothers.