Ichigo Mashimaro follows the life of Itou Nobue, her younger sister Chika, and her friends. The basic premise of the show can be summed up with "cute girls do cute things in cute ways", be it trying to quit smoking, going outside to play, celebrating a holiday, or doing school work.

It is the year 2301. Man has terraformed Mars into a beautiful water planet, and has renamed it “Aqua”. Built upon it is Neo-Venezia, an exact replica of old Venice, with beautiful waterways, palaces and plazas. Akari Mizunashi, a 15 year old girl arrives from Man-Home (formerly known as Earth) to become a gondolier trainee at a company called Aria… more It is the year 2301. Man has terraformed Mars into a beautiful water planet, and has renamed it “Aqua”. Built upon it is Neo-Venezia, an exact replica of old Venice, with beautiful waterways, palaces and plazas. Akari Mizunashi, a 15 year old girl arrives from Man-Home (formerly known as Earth) to become a gondolier trainee at a company called Aria Company. The company is lead by Alicia Florence, one of the three best gondoliers, also known as the three Water Fairies. Trough hard training, Akari aims to become a Prima herself. Many encounters and mysterious events are sure to follow on this planet of miracles

Life goes on for the ladies in the Yamayurikai. On the one-year anniversary of Sachiko and Yumi becoming sisters, Yumi receives a difficult task from Sachiko: to finally acknowledge someone as her younger sister.